Thermostabilities (T₉₀ values) of acute phase standard panel viruses (PSVs) of clades A, B and C, and lack of a relationship with susceptibility to soluble CD4.

<p>(<b>A</b>) Bar graph indicating the various T₉₀ values with that of the thermostable HIV-1_JR-CSF, and thermolabile HIV-1_ADA indicated with dashed lines. Env panel members that are thermostable (T₉₀≥48°C), intermediate in thermostability (43°C90<48°C), and thermolabile (T₉₀≤43°C) are indicated by filled, hatched and open bars, respectively. (<b>B</b>) Bell curve (normal distribution) in T₉₀ values of HIV-1 Envs used in this study, including Envs from acute phase panels of clades A, B and C (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021339#pone-0021339-t001" target="_blank">Tables 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021339#pone-0021339-t002" target="_blank">2</a>), n = 34. Mean, 44.2°C; Std. Dev., 2.4°C; Range, 40.0–49.0°C. The curve-fit (nonlinear regression lorentzian) was made using Prism software (Graphpad, CA). (<b>C</b>) Lack of correlation between T₉₀ and reported IC₅₀ values of soluble CD4 against HIV-1 standard Env panels (clades B and C). Soluble CD4 IC₅₀ data are taken from Wu et al <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021339#pone.0021339-Wu3" target="_blank">[79]</a>, excluding resistant isolates (IC₅₀>50 µg/ml) for which exact values are undetermined.