Plasma levels of total sAPP in control and autistic patients.

<p>Plasma levels of total sAPP (α and β) were assayed by Western blot from control and autistic patients. Autistic patients were classified as mild-to-moderate or severe based on clinical CARS score. Total sAPP bands were analyzed by scanning the final blot followed by densitometry, ImageJ quantification, and normalization against β-actin bands. Statistical significance was assessed using ANOVA. No significant differences observed in mean total sAPP levels between control or autistic patients.