PCR confirmation of deletions in Δ<i>bpsl2179</i> and Δ<i>bpss0711</i> mutants of <i>B. pseudomallei</i>.

<p>Primers BPSL2179-up and BPSL2179-down were used to generate a 3108 bp fragment from strains carrying the wild type <i>bpsl2179</i> allele or a 2100 bp fragment from stains carrying the Δ<i>bpsl2179</i> allele. Primers BPSS0711-up and BPSS0711-down were used to generate a 3247 bp fragment from strains carrying the wild type <i>bpss0711</i> allele or a 2188 bp fragment from strains carrying the Δ<i>bpss0711</i> allele. Lane labels and samples analyzed are as follows: MW, DNA ladder; Δ<i>bpss0711</i>, amplicon from strain carrying the Δ<i>bpsl2179</i> mutant allele; <i>bpss0711</i>, amplicon from strain carrying the wild type <i>bpss0711 allele</i>; Δ<i>bpsl2179</i>, amplicon from strain carrying the Δ<i>bpsl2179</i> mutant allele; and <i>bpsl2179</i>, amplicon from strain carrying the wild type <i>bpsl2179</i> allele.