<p>FtsZ (11 µM) was incubated in the polymerisation buffer (pH = 6.5) for 15 minutes prior to the addition of GTP (0.2 mM). At the indicated times the reaction was stopped and the samples were negatively stained by the addition of 1% uranyl acetate. Scale bar represents 0.2 µm for ×75,000 magnification and 100 nm for ×120,000 magnification.</p
There is a growing body of evidence that bacterial cell division is an intricate coordinated process...
There is a growing body of evidence that bacterial cell division is an intricate coordinated process...
<p>(<b>A</b>) Formation of GTP analysed on TLC. (<b>B</b>) Quantitation of the GTP formed. (<b>A</b>...
<p>(a) pH = 6.0, (b) pH = 6.5, (c) pH = 7, for 15 minutes prior to the addition of GTP (0.2 mM). The...
<p>FtsZ (11 µM) in polymerisation buffer (50 mM MES buffer pH 6.5, 10 mM MgCl<sub>2</sub> and 50 mM ...
<p>Purified wild-type and mutant FtsZ were polymerized in reactions containing 5 µM of FtsZ, Mes/NaO...
<p>FtsZ (5 µM) was polymerized by GTP (25 µM) in the absence (A) and presence of SB-P17G-A20 at 40 µ...
During bacterial cell division, the essential protein FtsZ assembles in the middle of the cell to fo...
<p>FtsZ (2 µM) was polymerized in 25 mM PIPES buffer, pH 6.8 containing 1 mM MgCl<sub>2</sub> and 1 ...
<p>The SDS-PAGE profile of the sample in the pellet (<b>A</b>) and in the supernatant (<b>B</b>). It...
During bacterial cell division, the essential protein FtsZ assembles in the middle of the cell to fo...
<p><i>A</i> and <i>B</i>. Normalized FCS autocorrelation profiles of FtsZ-GTP (12 μM) in the presenc...
<p>Top row, 10 μM EcFtsZ was polymerized at 10, 20 and 30°C (A-C). Bottom row, 10 μM MjFtsZ was poly...
<p>A) Polymerization of FtsZ with or without the addition of SlmA and SBS17-30mer. Reactions were pe...
<p>FtsZ (6 µM) was polymerized in the absence (○) and presence of 2 µM (•) and 4 µM (▪) of ZipA (Pan...
There is a growing body of evidence that bacterial cell division is an intricate coordinated process...
There is a growing body of evidence that bacterial cell division is an intricate coordinated process...
<p>(<b>A</b>) Formation of GTP analysed on TLC. (<b>B</b>) Quantitation of the GTP formed. (<b>A</b>...
<p>(a) pH = 6.0, (b) pH = 6.5, (c) pH = 7, for 15 minutes prior to the addition of GTP (0.2 mM). The...
<p>FtsZ (11 µM) in polymerisation buffer (50 mM MES buffer pH 6.5, 10 mM MgCl<sub>2</sub> and 50 mM ...
<p>Purified wild-type and mutant FtsZ were polymerized in reactions containing 5 µM of FtsZ, Mes/NaO...
<p>FtsZ (5 µM) was polymerized by GTP (25 µM) in the absence (A) and presence of SB-P17G-A20 at 40 µ...
During bacterial cell division, the essential protein FtsZ assembles in the middle of the cell to fo...
<p>FtsZ (2 µM) was polymerized in 25 mM PIPES buffer, pH 6.8 containing 1 mM MgCl<sub>2</sub> and 1 ...
<p>The SDS-PAGE profile of the sample in the pellet (<b>A</b>) and in the supernatant (<b>B</b>). It...
During bacterial cell division, the essential protein FtsZ assembles in the middle of the cell to fo...
<p><i>A</i> and <i>B</i>. Normalized FCS autocorrelation profiles of FtsZ-GTP (12 μM) in the presenc...
<p>Top row, 10 μM EcFtsZ was polymerized at 10, 20 and 30°C (A-C). Bottom row, 10 μM MjFtsZ was poly...
<p>A) Polymerization of FtsZ with or without the addition of SlmA and SBS17-30mer. Reactions were pe...
<p>FtsZ (6 µM) was polymerized in the absence (○) and presence of 2 µM (•) and 4 µM (▪) of ZipA (Pan...
There is a growing body of evidence that bacterial cell division is an intricate coordinated process...
There is a growing body of evidence that bacterial cell division is an intricate coordinated process...
<p>(<b>A</b>) Formation of GTP analysed on TLC. (<b>B</b>) Quantitation of the GTP formed. (<b>A</b>...