Survival of wild type and isogenic alanine racemase deficient mutant strains of <i>B. mallei</i> within murine macrophages.

<p>Murine peritoneal periodate-elicited macrophages were infected in vitro with wild type or alanine racemase deficient mutant strains of <i>B. mallei</i>. After 2 h of phagocytosis in d-alanine-supplemented RPMI⁺ medium, the macrophages were washed with prewarmed RPMI⁺ medium containing 6 µg/ml gentamicin with or without 5 mM d-alanine. The surviving intracellular bacteria were enumerated after 4 h of culture. The % survival was enumerated by (cfu t_n/cfu t₀)100.