Lung large cell carcinoma, hepatocarcinoma and colon adenocarcinoma.

<p>(<b>A</b>–<b>C</b>) Juxtanuclear PaCS in a lung large cell carcinoma (<b>A</b>, 10,000x), boxed area enlarged in <b>B</b> (50,000x) whose boxed area is enlarged in <b>C</b> (75,000x) to show its particulate structure and proteasome immunogold reactivity. (<b>D</b> and <b>E</b>) Hepatocellular carcinoma showing both hyaline bodies (hb), reactive for p62 protein in <b>D</b> (30,000x) and unreactive for 19S proteasome in <b>E</b> (25,000x), and PaCS (asterisks) unreactive for p62 and reactive for 19S proteasome. r, ribosomes. Inset to <b>D</b> (450x): hyaline and Mallory bodies react for p62 protein immunoperoxidase in formalin-fixed paraffin sections. (<b>F</b>–<b>H</b>) Colon adenocarcinoma (<b>F</b>, 10,000x) with partly intracellular and partly interstitial bacteria, a cytoplasmic vacuole (v) and a PaCS (boxed) bordering the cell plasma membrane, enlarged in <b>G</b> (40,000x) and <b>H</b> (75,000x). Note partial lysis of proteasome particles, though retaining 19S proteasome reactivity, development of irregular dense bodies and an encircling peripheral membrane, suggesting transition from a PaCS to an autophagic vacuole, also enclosing ribosome-like particles (r) and a bacterium (top right in <b>G</b> and <b>H</b>) with capsulated wall typical of Gram-positive organisms.