Senescence affects morphology and angiogenic potential of endothelial cells.

<p>A. Cell size in late (P12) passage cells is increased by 36% compared to early (P4) passage cells as judged by a number of cells within the field of view in confluent cultures, and the number of nuclei stained with DAPI in 100% confluent cultures is smaller in P12 cells. B. Graph depicts the length of tubes formed by P4, P8 and P12 cells on matrigel in the absence or the presence of 20 ng/ml VEGF. Values are expressed as fold change in relation to unexposed P4 Control. C. Representative Western blots for 4-HNE modified proteins in lysates from P4 and P12 HUVECs. D. Representative Western blots for VE-cadherin and von Willebrand factor, and E. Relative protein content in relation to P4 values; data in mean ± SEM from 3 independent experiments. (*) – p<0.05 compared to P4 cells; (†) – p<0.05 compared to the same passage Control.