FACS analysis of multilineage human hematopoiesis in humanized Rag1^−/−γc^−/− mice.

<p>Single cell suspensions were made from spleen, bone marrow, lymph node and thymus of humanized Rag1^−/−γc^−/− mice and were stained with different antibodies to detect human hematopoietic cell sub-sets. Human anti-CD45 antibody was used to define human leukocytes. From this population human CD3+ T cells (A) as well as CD4+ and CD8+ T cell subsets were identified in the lymph node, spleen and thymus (B). Dendritic cells were identified by their lack of lineage staining (CD3, CD19, CD14, CD16, CD20 and CD56) and expression of HLA DR (C). Both myeloid (CD11c) and plasmacytoid (CD123) dendritic cells (D) were identified in lymphoid organs as were CD14 expressing monocytes (E) and two subsets of CD19 and CD20 expressing B cells (F).