Rapid throughput assay showing influence of Ca²⁺availability on uptake of Zn²⁺in populations of isolated gastric glands, during exposure to secretagogues.

<p>Aliquots of fluozin-3 loaded glands (n = 8 each group) were incubated in wells for 45 min in Ca-Ringer's or 0Ca-Ringer's solutions under control conditions, during exposure to secretagogue combination (carbachol CCh 100 µM, forskolin FSK, 10 µM), or during exposure to secretagogue combination and thapsigargin TG, 10 µM. Glands were then exposed for 10 min to solutions containing Zn²⁺ 10 µM to monitor content. Results reported in total fluorescence units, means ±SEM, *p<0.05 compared to Ringer control, ¶p<0.05 compared to CaR conditions. <b><i>Designations:</i></b> a) Ca-Ringer's 45 min then Ringer's/Zn²⁺10 µM 15 min (<b>CaR</b>); b) 0Ca-Ringers 45 min/Zn²⁺10 µM 15 min (<b>0CaR</b>); c) Ca-Ringer's, carbachol 100 µM, forskolin 10 µM for 45 min then Ca-Ringer's, carbachol 100 µM, forskolin 10 µM, Zn²⁺10 µM 15 min (<b>CaR-CCh-FSK</b>); d) 0Ca-Ringer's/2 µM Thapsigargin 45 min then 0Ca-Ringer's/Thapsigargin 2 µM/Zn²⁺10 µM 15 min (<b>0CaR-CCh-FSK</b>); e) Ca-Ringer's, carbachol 100 µM, forskolin 10 µM, 2 µM, thapsigargin for 45 min then Ca-Ringer's, carbachol 100 µM, forskolin 10 µM, thapsigargin 2 µM/Zn²⁺10 µM 15 min (<b>CaR-CCh-FSK/TG</b>); f) 0Ca-Ringer's/2 µM Thapsigargin 45 min then 0Ca-Ringer's/Thapsigargin 2 µM/Zn²⁺10 µM 15 min (<b>0CaR-CCh-FSK/TG</b>).