Characteristics of megakaryocyte morphology in MPNs and controls (CTRL).

<p>(A) Representative picture of differently shaped CD41⁺ (red) cells of controls (I), pre-fibrotic PMF(II), PMF (III), ET (IV) and PV (V). Nuclei are counterstained with Hoechst 33258 (blue). Scale bars are 15 µm. (B) Ploidy was analyzed as described in Methods. PMF derived megakaryocytes presented lower polyploidy with respect to controls. (C) PMF derived CD41⁺ megakaryocytes (red) displayed bulbous and hypo-segmented nuclei.(blue) (left panel). Scale bar is 15 µm. Means ± SD of the percentage of megakaryocytes displaying abnormal nuclei in pre-fibrotic PMF and PMF, as compared to control, are reported in the right panel. (D) Diameters of MPN derived CD41⁺ megakaryocytes (red) were analyzed as shown in the left panel and were performed as described in Methods. Scale bar is 10 µm. For each MPN category at least 100 megakaryocytes were analyzed, the means ± SD of diameters is reported in the right panel. *p<0.05.