Ultrasonic vocalizations in the presence of female urine in adult male <i>Shank1</i> mice.

<p>(A) Total number of ultrasonic vocalizations emitted during the 5 min test session in the open field containing urine from a female C57BL/6J mouse by male subjects before they had an experience of social interactions with a female. (B) Total number of ultrasonic vocalizations emitted during the 5 min test session in the open field containing urine from a female C57BL/6J mouse by male subjects 7 days after they had a 5 minute experience of social interactions with a female. (C) Time course for the number of ultrasonic vocalizations emitted for each 10 s time bin across the 5 min test session with exposure to female urine before female experience. (D) Time course for the number of ultrasonic vocalizations emitted for each 10 s time bin across the 5 min test session with exposure to female urine after female experience. Black bar: <i>Shank1^+/+</i> wildtype littermate control mice; striped bar: <i>Shank1^+/</i>⁻ heterozygote mice; white bar: <i>Shank1</i>^{−<i>/</i>−} null mutant mice. For the sake of clarity, <i>Shank1^+/</i>⁻ heterozygote mice were not included in the time course graphs, while still included in the statistical analysis. Data are presented as means ± standard errors of the mean. * p<0.050 vs. <i>Shank1^+/+</i>; # p<0.050 vs. <i>Shank1^+/</i>⁻.