Screening of circular-permutated mCherry variants in <i>E.coli</i>.

*<p>Circular-permutated mCherry variants were numbered by the primary amino acid sequence of mCherry as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020505#pone-0020505-g001" target="_blank"><b>Fig. 1C</b></a>. Variants are labeled with the new amino and carboxy termini (e.g. cp159-158 has the new N-terminus from the native carboxy amino acids 159 to 236 and the new C-terminus from the amino residues 1 to158).</p>a<p>Fluorescent variants from the initial screening in each region.</p>b<p>Aligns to cp-EGFP in GCaMP2.</p>c<p>Numbered by the amino acid sequence of DsRed as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020505#pone-0020505-g001" target="_blank"><b>Fig. 1C</b></a><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020505#pone.0020505-Li1" target="_blank">[18]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020505#pone.0020505-Carlson1" target="_blank">[19]</a>. cp184-mCherry corresponds to cp191-190, with three duplicated residues at N-terminus (188-190). The cp193-mCherry and cp193g7 (with 6 mutated amino acids) correspond to cp197-196, with three duplicated residues (197-199) at the C-terminus.