Activity of heat shock promoters in the <i>C. elegans</i> germline.

<p>(A) MosSCI strains generated for heat shock experiments. (B) Time course analyses of <i>Phsp-16.41/gfp;:his-58/tbb-2 3′UTR</i> (strain EG5295). Different shades of gray indicate rough quantification of average intensity levels of signals observed at indicated time points after heat shock. Darker shades indicate a stronger signal. Data were collected in two independent experiments observing 7–13 samples per stage at each time point; embryos were assessed starting from 3 h of recovery. Regions scored are shown in (C–E). (C) GFP-HIS-58 fluorescence observed close to the loop region of the gonad at 1 h after recovery from heat shock. (D) Fluorescence in oocyte nuclei (stars) at 4 hours post heat shock. (E) Fluorescence in embryonic nuclei at 4.5 h after heat shock.