Long-term Continuous CORT treatment increases VEGF protein levels <i>in vitro</i> and <i>in vivo</i>.

<p>(<i>A</i>) CORT (CORT; 1 µM) was applied to mouse primary cortical neurons at DIV 5. VEGF protein levels were determined by western blotting analysis at 48 hand 72 h following CORT treatment. CON means DMSO treatment. Data represent mean±SE (<i>n</i> = 6) expressed as fold change in VEGF protein levels as compared to CON. *<i>P</i><0.05 (Bonferroni's test). (<i>B</i>) VEGF protein levels in frontal cortex of mice treated with CORT (5 mg/kg) or vehicle control (CON; 0.45% hydroxypropyl-β-cyclodextrin) for 7 weeks were determined by western blot analysis. Data represent mean±SE (<i>n</i> = 5) expressed as fold change in VEGF protein levels as compared to CON. *<i>P</i><0.01 (t test). <i>(C)</i> VEGF protein levels in serum samples collected from mice treated with CORT (CORT; 5 mg/kg) or vehicle control (CON; 0.45% hydroxypropyl-β-cyclodextrin) for 7 weeks were analysed by ELISA. Data represent mean±SE (<i>n</i> = 5–6) expressed as fold change in VEGF protein levels as compared to CON. *<i>P</i><0.01 (t test).