PI3K signaling is not involved in long-term continuous CORT-induced increases in VEGF protein levels.

<p>(<i>A</i>) VEGF protein levels were determined by western blotting analysis in neuronal cell lysates treated with LY294002 (LY; 20 µM) for 48 h. CON means DMSO treatment. Data represent mean±SE (<i>n</i> = 6) expressed as fold change in VEGF protein levels as compared to CON. *<i>P</i><0.05 (Bonferroni's test). (<i>B</i>) Pretreatment with LY did not prevent CORT-induced induction in VEGF protein levels in neurons. Cortical neurons at DIV 5 were treated with LY (20 µM) for 30 min followed by CORT (1 µM) exposure for 48 h. VEGF protein levels were determined in cell lysates by western blot analysis. CON means DMSO treatment. Data represent mean±SE (<i>n</i> = 6) expressed as fold change in VEGF protein levels as compared to CON. *<i>P</i><0.05 (Bonferroni's test).