Ricin toxicity and antibody neutralising effect <i>in vitro</i> using a viability assay with Jurkat cells.

<p>(<b>A</b>) Evaluation of ricin toxicity with Jurkat cells. Ricin (0–100 ng/ml) was incubated with 2×10⁴ cells/ml and cell viability was assessed by means of luminescence assay using the Cell titer Glo luminescence kit (Promega). (<b>B</b>) Neutralisation assay of ricin using anti-A chain antibodies (RA30: •; RA35: ▪; RA36: ▴; RA32: ▾ and RA33: ♦). (<b>C</b>) Neutralisation assay of ricin using anti-B chain antibodies (RB18: ◊; RB27: Δ; RB34: ○ and RB37: □). For Figures (B) and (C), 0.1 ng/ml ricin was pre-incubated with 0–10 µg/ml antibody and then exposed to 2×10⁴ cells/ml for 72 h before assessment of cell viability in the same way as in Figure (A).