Broad-range real-time PE-PCR and HRM analysis for 12 different bacterial species.

<p>The indicated amounts of genomic DNA from 12 different bacterial species were subject to broad-range real-time PE-PCR using the fusion probe M13-16S-p201F and the primer set M13 and p1370 in the presence of LCGreen I plus. The PCR product was subject to HRM analysis using HR-1 instrument and the derivative plots were shown. NTC, no template control.