Figure 2

<p>(A) Quantitative assessment of the extent of myonecrosis and regeneration in mouse gastrocnemius muscle injected with either <i>B. asper</i> venom, Mtx or BaP1. Histological sections stained with hematoxylin and eosin were analyzed one and 28 days after injections. The extent of necrosis was estimated in samples collected one day after injection as the percentage of the examined area corresponding to necrotic fibers, whereas the extent of regeneration was estimated in samples collected at 28 days as the percentage of the examined area corresponding to regenerating fibers, i.e. fibers having centrally-located nuclei. *p < 0.05 when comparing the percentage of necrosis and of regeneration for a single treatment. (B) Quantitative assessment of the diameter of regenerating muscle fibers, i.e. fibers presenting centrally-located nuclei, 28 days after intramuscular injection in the gastrocnemius of PBS, <i>B. asper</i> venom, Mtx or BaP1. Regenerating fibers in tissue injected with venom and BaP1 showed a reduced diameter when compared with control fibers in tissue injected with PBS (p < 0.05), whereas no significant difference was observed in the diameter of regenerating fibers in muscle injected with Mtx (p > 0.05). In both graphs, results are presented as mean±SD (n = 9).