Joint-dated phylogeny of the AMPK subunits.

<p>(A) subunit α; (B) subunit β; (C) subunit γ. Sequences obtained in this study are in bold and green; mis-annotated database sequences (NCBI) are in gray. Posterior probabilities are indicated only when <0.70 (all the others are ≥0.70). Error bars (95% Highest Posterior Densities or HPDs) are given only for two genome-wide duplication (GWD) events when these are still visible by the pattern of paralogs: the second round (GWD-2) in vertebrates and the fish-specific event (GWD-3). Mean posterior estimates are indicated by “+” signs. The 95% HPDs of these GWD events are shaded in red and blue, respectively and were constructed from the union of HPDs taken over all detected paralogs across all three subunits. TSA: Transcriptome Shotgun Assembly.