Expression of PKDs in taste tissues.

<p><b>A.</b> mRNA expression of PKD1L3 and PKD2L1 in taste tissues of WT, PKD1L3^−/−, PKD2L1^−/−, PKD1L3/2L1^dbl−/−, GAD-GFP, GAD-GFP+PKD1L3^−/−, and GAD-GFP+PKD2L1^−/− mouse. FP: fungiform taste buds. CV: circumvallate taste buds. ET: epithelial tissue. Gustducin is a control for taste tissue. β-actin is an internal control. 100 bp marker was used. <b>B</b>, <b>C.</b> Immunostaining for PKD1L3 (<b>B</b>) and PKD2L1 (<b>C</b>) in taste tissues of GAD-GFP+WT mouse. <b>D.</b> Immunostaining for PKD1L3 in taste tissues of GAD-GFP+PKD1L3^−/− mouse. <b>E.</b> Immunostaining for PKD2L1 in taste tissues of GAD-GFP+PKD2L1^−/− mouse. <b>F.</b> Immunostaining for GAD67 in taste tissue of GAD-GFP+WT mouse. <b>G.</b> Immunostaining without primary antibody in GAD-GFP+WT mouse. Green shows GFP fluorescence. Red shows immunoreactivity (IR) for PKD1L3, PKD2L1 or GAD67, respectively. FP: fungiform papillae. CV: circumvallate papillae. Scale bar, 10 µm.