<i>Grp94</i> KO HSCs failed to reconstitute the hematopoietic system in the presence of WT competitors.

<p>A) Scheme of the <i>in vivo</i> competitive repopulation assay. B) Representative flow cytometric analysis with tail blood from recipient mice 4 weeks after BM transplantation. CD45.1⁺ cells represent blood cells from WT competitor, while CD45.2⁺ cells represent progenies from WT or cKO LSK cells. Tail blood from recipient mice 8, 12, and 24 weeks after BM transplantation yielded the same results (data not shown). C) Summary of the percentage contribution of WT or cKO LSK cells to the peripheral blood MNCs (n = 5). The data are presented as mean ± s.e., ***p<0.001.