<p>(<b>A</b>) Spleen cells were obtained from vaccinated and non-vaccinated dogs at one year after challenge infection with <i>T. cruzi</i>. Spleen cells were incubated for 30 min with FITC-conjugated anti-CD8 and PE-conjugated anti-CD4 antibodies and CD4<sup>+</sup> and CD8<sup>+</sup> T cell subsets monitored by flow cytometry. (<b>B</b>) The circulatory IFN-γ level was measured by an ELISA. Data are presented as mean ± SD (*<i>p</i><0.05).</p
<p>Thirty days after vaccination, lung and spleen suspensions were stimulated <i>ex</i><i>vivo</i> w...
<p><sup>a</sup> Number of IFNγ secreting cells/10<sup>6</sup> splenocytes (splenocytes were removed ...
<p>PBMC from dogs vaccinated with Ech_0660 and challenged with wild-type <i>E</i>. <i>chaffeensis</i...
<p>Dogs were vaccinated with TcVac4 or TrIE only and infected with <i>T</i>. <i>cruzi</i>, as descri...
International audienceVaccines to prevent Trypanosoma cruzi infection in humans or animals are not a...
<p>CD8<sup>+</sup> T cell proliferation and IFNγ production were measured using similar approaches a...
<p>(A-B) Negative control (suppression of primary antibody). Immunostaining of (C-D) CD3<sup>+</sup>...
<p>Peripheral blood cultures were collected after 7 days in culture with recombinant canine IL-4 and...
<p>Mice were injected with empty vector or TcVac3 (± cytokine adjuvants), infected with <i>T. cruzi,...
<p>Mice were immunized with empty vector or TcVac3 (± cytokine adjuvants), and infected with <i>T. c...
<p>Dogs were vaccinated as above, and infected with <i>T. cruzi</i>. An ELIS...
<p>Mice were immunized as in Fig. 1, and harvested two-weeks after last immunization. (<b>A</b>) Spl...
(A) The frequency of canine CD4+ single-positive (sp), CD8α+ sp, and CD4+CD8α+ dp T cells in differe...
<p>PBMCs were obtained from dogs at the time of diagnosis (pre) and 3 weeks post vaccination (post) ...
The Leishmune (R) vaccine has been used in endemic areas to prevent canine visceral leishmaniasis in...
<p>Thirty days after vaccination, lung and spleen suspensions were stimulated <i>ex</i><i>vivo</i> w...
<p><sup>a</sup> Number of IFNγ secreting cells/10<sup>6</sup> splenocytes (splenocytes were removed ...
<p>PBMC from dogs vaccinated with Ech_0660 and challenged with wild-type <i>E</i>. <i>chaffeensis</i...
<p>Dogs were vaccinated with TcVac4 or TrIE only and infected with <i>T</i>. <i>cruzi</i>, as descri...
International audienceVaccines to prevent Trypanosoma cruzi infection in humans or animals are not a...
<p>CD8<sup>+</sup> T cell proliferation and IFNγ production were measured using similar approaches a...
<p>(A-B) Negative control (suppression of primary antibody). Immunostaining of (C-D) CD3<sup>+</sup>...
<p>Peripheral blood cultures were collected after 7 days in culture with recombinant canine IL-4 and...
<p>Mice were injected with empty vector or TcVac3 (± cytokine adjuvants), infected with <i>T. cruzi,...
<p>Mice were immunized with empty vector or TcVac3 (± cytokine adjuvants), and infected with <i>T. c...
<p>Dogs were vaccinated as above, and infected with <i>T. cruzi</i>. An ELIS...
<p>Mice were immunized as in Fig. 1, and harvested two-weeks after last immunization. (<b>A</b>) Spl...
(A) The frequency of canine CD4+ single-positive (sp), CD8α+ sp, and CD4+CD8α+ dp T cells in differe...
<p>PBMCs were obtained from dogs at the time of diagnosis (pre) and 3 weeks post vaccination (post) ...
The Leishmune (R) vaccine has been used in endemic areas to prevent canine visceral leishmaniasis in...
<p>Thirty days after vaccination, lung and spleen suspensions were stimulated <i>ex</i><i>vivo</i> w...
<p><sup>a</sup> Number of IFNγ secreting cells/10<sup>6</sup> splenocytes (splenocytes were removed ...
<p>PBMC from dogs vaccinated with Ech_0660 and challenged with wild-type <i>E</i>. <i>chaffeensis</i...