<i>In vivo</i> analysis of the Dominant Negative phenotype of Cry mutants.

<p>Panel A, Toxicity assays against <i>Manduca sexta</i> larvae of different Cry toxins at their corresponding LC₅₀ concentration (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019952#pone-0019952-t001" target="_blank">table 1</a>) (black bars) and mixtures of Cry1Ab-D136N/T143D mutant with these wildtype Cry toxins in a protein ratio 0.5∶1 (mutant∶wildtype) (white bars). Panel B, Toxicity assays against <i>Manduca sexta</i> or <i>Aedes aegypti</i> larvae of Cry1Ab or Cry11Aa at their corresponding LC₅₀ concentration (black bar) or in a mixture with Cry1Ab-R99E or Cry11Aa-E97A mutant toxins in a protein ratio 1∶1 (dashed bars), 10∶1 (mutant∶wildtype) (grey bars) or 100∶1 (mutant∶wildtype) (white bars). Panel C, Toxicity assays against <i>Aedes aegypti</i> larvae of Cry4Ba or Cry11Aa at their corresponding LC₅₀ concentration (black bar) or in a mixture with mutant toxins in a protein ratio 0.5∶1 (mutant∶wildtype) (white bars) or 1∶1 (dashed bars).