Cytotoxic activity of CD8⁺ T lymphocytes activated by MVA-B-infected MDDC.

<p>Effector cells were autologous monocyte-depleted PBMC co-cultured for 6 days with MDDC mock-treated or infected with MVA or MVA-B. After this co-culture period, CFSE staining of effector cells was performed and then were cultured for 4 h with target cells at a 10∶1 ratio. Target cells were autologous CD8-depleted T cells infected with a primary HIV-1 isolate and their corresponded to the phenotype CFSE-negative, CD3-positive CD8-negative. In (A) the flow cytometry analysis is shown. In (B), the mean (±SEM) count of target CD4⁺ T cells is a representative result of three independent experiments (performed in triplicates) is depicted. (*) p<0.05.