Competitive experiments between bovine wt-RV and human r-RV.

<p>Competitive experiments were performed by co-infecting MA-104 cells by the bovine wt-RV RF and one of the human r-RV at an MOI ratio of 1∶1 (0.3 PFU/cell for each virus). The resulting cell culture lysates were serially propagated in MA-104 cells. RNA profiles of viral progenies resulting from mixed infections by wt-bovine RF virus and human r-RV M1 (A), M3 (B), M4 (C), or M2 (D). RNA profiles of wt-bovine RF and human r-RV show differences of mobility for 8 RNA segments (segments 1, 4–6, 8–11). I and Pn indicate the initial inoculum used for mixed infections and the passage number, respectively. Numbers indicate the location of RNA segments. Arrows indicate rearranged segments 7R, 7RΔ, and 11R.