Level of expression of the wild-type and truncated αA-Crystallin constructs in HeLa cells.

<p><b><i>A:</i></b><i> Western blot analysis of individual expression of αA- constructs in HeLa cells</i>. Cells were transfected with a total of 2 µg of YFPαAwt, YFPαA162, YFPαA168 and YFPαA172 constructs. After 48 hours transfection, cells were lysed and subjected to immunoblot and probed with anti-αA-Crystallin antibody. This antibody is specific for αAwt and the truncated αA-crystallins, which recognizes a 46 kDa (the mass of αA-crystallin+YFP) protein. The same blot was stripped and re-probed with anti-β-actin for loading controls. <b><i>B:</i></b><i> Quantitative data for western blot analysis as in </i><i>Fig. 2A</i>. Quantitation of αA-Crystallin wild-type and truncated constructs normalized against β-Actin was determined with NIH Image J Software. The levels were averaged over three independent experiments and plotted. The values are means ± Standard Deviation.