Competitive experiments between reassortant RF7RΔ and wt-RF viruses at an MOI ratio of 1∶10⁷.

<p>Competitive experiments were performed by co-infecting MA-104 cells by 3×10⁻⁷ PFU/cell of RF7RΔ and 3 PFU/cell of wt-RV RF (1∶10⁷ MOI ratio). The resulting viral progeny was serially propagated in MA-104 cells and viral dsRNA was analyzed by PAGE (A) and RT-PCR for specific detection of rearranged segment 7 (B). Pn indicates the passage number; arrows indicate the location of segment 7RΔ and the expected size of the PCR product (466 bp).