Proliferation rates in fibroblasts pretreated with kinase inhibitors.

<p>4×10³ cells/well were pretreated with the p38 MAPK inhibitor SB202190, AKT inhibitor LY294002, or ERK1/2 inhibitor PD98059 for 1 h and then seeded in a 96-well plate containing either native collagen or 3DG-collagen for 0 h, 24 h, and 48 h. At each designated time point the level of proliferation was determined using the cell proliferation reagent WST-1. Quantification of each sample was determined by measuring the absorbance at 450 nm, with a reference wavelength of 600 nm. Comparisons were performed within each time point and compared to their respective controls (native collagen or 3DG-collagen). Data are mean ± SD (n = 3), **P<0.01, *P<0.05.