Effect of Plumbagin on Colonic cancer cells and normal PBMCs.

<p><i>a. Cytotoxicity effect of various concentrations of Plumbagin on HCT15 cells</i>. Dose dependent cytotoxicity effects of Plumbagin on HCT15 cells were represented in the above graph. HCT15 cells were more sensitive to plumbagin as IC₅₀ at 24 hours was 22.5 µM. All the experiments were done in triplicates and expressed as the mean ± SD. Significance is indicated as *<i>p<0.001</i>. <i>b. Cytotoxicity effect of various concentrations of Plumbagin on HT29 cells</i>. Dose dependent cytotoxicity effects of Plumbagin on HT29 cells were represented in the above graph. HT29 cells were more sensitive to plumbagin as IC₅₀ at 24 hours was 62.5 µM. All the experiments were done in triplicates and expressed as the mean ± SD. Significance is indicated as *<i>p<0.001</i>. <i>c. Cytotoxicity effect of various concentrations of Plumbagin on PBMC cells</i>. Dose dependent cytotoxicity effects of Plumbagin on PBMC cells were represented in the above graph. PBMC cells were resistance to plumbagin induced cytotoxicity even at 100 µM concentration. All the experiments were done in triplicates and expressed as the mean ± SD. Significance is indicated as *<i>p<0.001</i>.