Induction of neurogenesis in P19 EC cells by RA.

<p>P19 cells were differentiated using embryoid bodies in the presence of RA as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019174#pone.0019174-JonesVilleneuve1" target="_blank">[35]</a>. (<b>A</b>): Formation of Tuj1-, and NF68- positive cells with neuronal morphology on day 6 and GFAP-positive cells with astrocyte morphology on day 10 of RA-induced (+RA) differentiation. Nuclei were stained with Hoechst, scale bar is 30 µM. (<b>B</b>): P19 EC cells fail to form Tuj1-, NF68- and GFAP- positive cells in the absence of RA (-RA) on the days indicated. Nuclei were stained with Hoechst, scale bar is 30 µM. (<b>C</b>): Tuj1-, NF68- and GFAP-positive cells from (A–B) were counted in 10 random fields and expressed as % of the total number of nuclei (3,000 nuclei). (<b>D</b>): The temporal pattern of expression of indicated genes during P19 EC differentiation +/−RA. Representative QPCR analysis is shown in which fold changes are relative to day 0. Error bars represent +/− SEM.