PTEN aptamers and PTEN detection in cerebellar histologies.

<p>Staining pattern in histologic samples of mouse cerebellum at two magnifications, 100× and 1000×, as indicated at the top of each column. To the left of each pair of panels the aptamer or antibody utilized for the histological staining assay of the PTEN protein was denoted. The layers of the cerebellum are indicated within each illustration as internal granular layer (IGL), Purkinje cell layer (PcL) and molecular layer (ML). The Purkinje cells are shown at the highest magnification, 1000×, where nuclear (N) or cytoplasmic (C) localization is distinguishable. The known aptamer Anp32e (Anp) was used as a positive control. The patterns belonging to clones 3, 4, 5, 6 and 9 corresponded to the aptamers developed against the recombinant protein GST-PTEN, while the patterns associated with z7 and z14 were the aptamers PTENz7 and PTENz14 developed against the peptides.