Expression of Gli/EnR delays neurogenesis and decreases gliogenesis in P19 EC cells.

<p>Cells were differentiated using a monolayer procedure described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019174#pone.0019174-Slack1" target="_blank">[46]</a> in the presence of RA. (<b>A–D</b>): Day 3 or day 6 differentiated P19[Gli/EnR] and P19 cells were stained with Tuj1- or NF68-specific antibodies. (<b>E</b>): Day 7 differentiated P19[Gli/EnR] and P19 cells were stained with GFAP-specific antibodies. Nuclei were stained with Hoechst, scale bar is 30 µM. (<b>F–H</b>): Tuj1-, NF68- and GFAP-positive cells from (A-E) were counted in 10 random fields and normalized with the number of nuclei (10,000 cells; n = 4), *p<0.05, **p<0.01, n.s. = not significant.