Effect of morphine on p38 MAPK and MEKK3/6 activation.

<p>WT, TLR9 KO, and µOR KO microglial cells were treated with morphine at 5 and 10 µM for 24 hr. (A) Total and phosphorylated p38 MAPK (p-p38 MAPK) levels were determined by Western blot. Mean values were derived from three independent experiments. * <i>p</i><0.01 compared with indicated groups. (B) Total and phosphorylated MKK3/6 (p-MKK3/6) levels were examined by Western blot. Mean values were derived from three independent experiments. * <i>p</i><0.01 compared with indicated groups.