Regulation of Runx2 promoter activity by TIEG1 in osteoblasts.

<p>(<b>A</b>) Putative krüppel-like transcription factor binding sites located within the −600 bp fragment of the Runx2 P1 promoter as identified using the Genomatix software suite. Krüppel-like transcription factor (KLTF), mouse krüppel like factor (MKLF), krüppel like zinc finger (KLZF) and C2H2 zinc finger containing transcription factor (C2H2) binding sites are indicated. (<b>B</b>) U2OS cells were transiently transfected with indicated control, full-length TIEG1 (1–480) or truncated TIEG1 (1–370) expression vectors and the full-length Runx2 promoter (−600) fused to a luciferase reporter. (<b>C</b>) U2OS cells were transiently transfected with indicated control or Runx2 promoter constructs fused to a luciferase reporter along with the full-length (1–480) TIEG1 expression construct. Twenty four hours post-transfection, luciferase activity was monitored and values are reported as relative fold change compared to controls following normalization to total protein levels. <i>Asterisks</i> denote significance at the p<0.05 level (ANOVA) compared with control values. δ denotes significance at the p<0.05 level (ANOVA) between indicated promoter constructs.