Egr-1 binds to Rad promoter after PDGF treatment.

<p>ChIP assay was performed in RASMC stimulated with or without PDGF (20 ng/ml) for 1 hour. After formaldehyde cross-linking, the protein-DNA complexes were recovered using anti-Egr-1 antibody or non-specific IgG. PCR was performed to detect the proximal Rad promoter. PCR products were detected by 2% agarose gel electrophoresis.