Increased oxidative damage to lipids in selected brain regions with age.

<p>(A) Liver, (B) heart, (C) kidney, and (D) lung were analysed by Western blotting using anti-4HNE antibody. The blots shown are representative tracings of an experiment done eight times. Graphs are mean ± S.E from tissue obtained from eight different rats for each age group. Each bar of the quantification graph represents the corresponding band for each age group. Significance ^*p<0.01 compared to 3 month old rats. (E) Immunodetection of 4-HNE in the liver, heart, kidney, and lung from 3 month, 12 month and 24 month old rats. 4-HNE (green) and DAPI (blue). Higher immunoreactivity for 4-HNE was observed in 12 and 24 month old rats compared to 3-month old rats.