Tim-3 expression on HTLV-1-specific CD8+ T cells in HTLV-1 infection.

<p>PBMC from HLA-A*02+ chronically HTLV-1 infected individuals were stained with matched HLA pentamers presenting CMV and HTLV-1 epitopes, and with an anti-Tim-3 antibody. Shown are representative flow cytometry data from one HTLV-1-infected person using HLA-A*02 pentamers presenting the (A) HTLV-I-Tax 11–19 epitope and, (B) CMV-pp65 epitope ‘NLVPMVATV’. (C, D) Plots show co-expression of Tim-3 (upper panel) and PD-1 (lower panel) with the respective HLA-A*02 pentamers (Tax (left) and CMVpp65 (right)) from the gated CD8+ T population depicted in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001030#pntd-0001030-g002" target="_blank">Fig 2</a> A, B. The percentages of cells in the upper left and right quadrants of the flow plots demonstrated in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001030#pntd-0001030-g002" target="_blank">Figure 2C, D</a> reflect only the percentage of pentamer expressing cells. The compiled expression data of the frequency of Tax (E) and CMVpp65 (F) pentamer cells on either Tim-3+ or Tim-3- and PD-1+ or PD-1- CD8+ T cells from 8 subjects are shown in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001030#pntd-0001030-g002" target="_blank">Figure 2</a> E and F. Statistical analyses comparing pooled responses were performed using the Mann-Whitney test.