LANA binds to the IL-22R1 promoter in vitro.

<p>(<b>A</b>) The aligned sequences of DNA fragments from TR DNA, wild type and mutant IL-22R1 promoters which contain LBS, the wild type LBS-like sequence and scrambled-mutant DNA sequence (underlined) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019106#pone.0019106-Garber2" target="_blank">[38]</a>. The nucleotide sequences represent the probe sequences used in the EMSA and were labeled with biotin. (<b>B</b>) LANA can bind to the LBS-like DNA sequence in IL-22R1 promoter. Probes as indicated were incubated for 20 min with or without purified LANA-C (a.a. 951–1162). NC, a DNA fragment derived from the sequence in M13 DNA, used as a negative control for LANA binding. (<b>C</b>) The binding of LANA to the wild type LBS-like sequence is specific. A 10-fold excess of cold competitor or mutant competitor DNA was added to compete the reaction between WT LBS-like and LANA. The upper and lower arrows indicate the LANA-specific binding band and free probe.