Analyses of GC and GS levels in the spleen and lung by LC/MS.

<p>(A) Spleen GC level in 4L;C* mice was not different from that in the WT. GS level was accumulated in 4L;C* spleen. By 600 mg/kg/d IFG treatment, GC or GS were increased by 2-fold in 4L;C* spleen at 14 day or 28 day, respectively. (B) GC and GS were accumulated in 4L;C* lung. IFG treatment did not reduce the substrate level in 4L;C* lung. Increased GC level was in 600 mg/kg/d IFG treated lung at terminal stage (Age was same as spleen in Fig. 5A). The data represent the mean±S.E. and analyzed by Student's t-test. *, p<0.05 (n = 3 mice). The GC and GS levels were normalized by tissue weight.