siRNA knockdown of Tcf3 in <i>Blm</i> wild type cells.

<p><b>A</b>. qRT-PCR analysis of <i>Tcf3</i> knockdown in <i>Tcf3</i> siRNA treated ES cells and control siRNA treated cells. <b>B</b>. Graph shows population of Rex1-EGFP positive cells in <i>Tcf3</i> siRNA and control siRNA treated cells after 2 days in monolayer differentiation with or without serum. <b>C</b>. qRT-PCR analysis of <i>Rex1</i> expression in <i>Tcf3</i> siRNA or control siRNA treated cells in monolayer differentiation with or without serum. <b>D</b>. Images showing a typical AP positive ES cell colony formed in <i>Tcf3</i> siRNA treated cells after 5 days in serum while only differentiated colonies formed from control siRNA treated cells. Error bar represents standard deviation from three individual plating.