Increased S6K1 activity in aortas of old rats is inhibited by resveratrol.

<p>(<b>A</b>) SA-ß-gal staining of the endothelium in an aorta segment of a young and an old WKY rat. (<b>B</b>) Immunoblotting analysis of S6-S235/S236 (p-S6) and total S6 in the aortas of young and old rats (n = 9 of each group). (<b>C</b>) The aortas of old rats were treated <i>ex vivo</i> with solvent as control (C), resveratrol (Resv, 10 µmol/L) or rapamycin (Rapa, 20 ng/ml) in Krebs-Ringer bicarbonate solution (37°C, 95% O₂/5% CO₂) for one hour. The lysates were prepared for Immunoblotting analysis of S6-S235/S236 (p-S6) and total S6 levels (n = 3). *p<0.05 and **p<0.01 between indicated groups.