Inhibition of S6K1 suppresses superoxide generation in the aortas of old rats.

<p>Confocal microscopic <i>en face</i> detection of MitoSox staining for mitochondrial superoxide anion production followed by counterstaining with DAPI for endothelial nuclei in the intact thoracic aorta segments from young and old rats, which were treated with resveratrol (Resv) or rapamycin (Rapa) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019237#pone-0019237-g010" target="_blank">Fig. 10</a>. The upper panels show representative images from a young and an old rat and lower panel shows quantification of the fluorescence intensity from three animals in each group. Scale bar = 50 µm. ***p<0.001 between indicated groups.