Laminin binding by non-α-DG glycoproteins in LARGE overexpressing DG-deficient cells was blocked by IIH6C4.

<p>IIH6C4 antibody was added to the culture medium of neural stem cells, laminin was added, and bound laminin was detected by immunofluorescence staining. (A, C, E, and G) Wildtype neural stem cells. (B, D, F, and H) DG-deficient neural stem cells. (A', C', E', and G') Wildtype neural stem cells treated with IIH6C4. (B', D', F', and H') DG-deficient neural stem cells treated with IIH6C4. (I) Quantification of bound laminin. Scale bar in H': 50 µm.