Inhibition of S6K1 increases NO production in the aortas of old rats.

<p>(<b>A</b>) Confocal microscopic <i>en face</i> detection of DAF-2DA staining for endothelial NO production followed by counterstaining with DAPI for endothelial nuclei in the intact thoracic aorta segments from young and old rats, which were treated with resveratrol (Resv) or rapamycin (Rapa) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019237#pone-0019237-g007" target="_blank">Fig. 7</a>. Shown are representative images from a young and an old rat. (<b>B</b>) Quantification of the fluorescence intensity from three animals in each group. Scale bar = 250 µm. ***p<0.001 between indicated groups. (<b>C</b>) Immunoblotting analysis of eNOS protein levels in aortas of young and old rats. Quantification of the signals is shown in the lower panel. n = 5, **p<0.01 vs. young rats.