Inclusion bodies formation of XynA expressed from plasmids pHsh-<i>xynA</i>2 and pET-<i>xynA</i>2.

<p><b>A–B</b>. Electronic microscopy observation of inclusion bodies in the pHsh-<i>xynA</i>2 transformed <i>E. coli</i> cells (A) and pET-<i>xynA</i>2 transformed <i>E. coli</i> cells (B), ultrathin section. <b>C</b>. SDS-PAGE analysis of XynA expressed from pET-<i>xynA</i>2. Lanes: M, protein marker; 1, total protein of <i>E. coli</i> containing pET-20b(+) without target gene; 2, total protein and 3, soluble protein of <i>E. coli</i> containing pET-<i>xynA</i>2 grown at 37°C; 4, total protein and 5, soluble protein of <i>E. coli</i> containing pET-<i>xynA</i>2 grown at 20°C.