Liver-specific PKA inhibition does not alter fasting-regulated gene expression.

<p>mRNA levels of enzymes required for gluconeogenesis in the 24 hr fasted state (Fast) or after being fasted for 24 hr then allowed access to food for 6 hr (Fed) in WT, RIαB<b>,</b> and RIαB/Alb-<i>cre</i> mice. Levels of each mRNA were measured by quantitative real time PCR as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018772#s4" target="_blank">Materials and Methods</a>. Data are expressed relative to the results of a fasted WT control animal. Each value represents the mean ± SEM; n = 3 animals per group. No significant differences were found in gene induction. PGC-1α (peroxisome proliferator-activated receptor-γ coactivator 1α), PEPCK (phospho<i>enol</i>pyruvate carboxykinase), G6Pase (glucose-6-phosphatase), GCK (glucokinase), GAPDH (glyceraldehyde 3-phosphate dehydrogenase), and GLUT2 (glucose transporter type 2).