Anti amastigote activity and safety index of Berberine chloride.

<p>The anti-leishmanial activity of Berberine chloride (0–25 µM, 72 h) was tested in intracellular amastigotes as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018467#s2" target="_blank">Methods</a>. Each point corresponds to the mean ± SD of at least three experiments in duplicate. <b>Inset</b>: The effect of Berberine chloride (0–100 µM) on viability of murine macrophages was evaluated at 48 h (▪), 72 h (▴) and 96 h (▾) by the MTS-PMS assay as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018467#s2" target="_blank">Methods</a>. Each point corresponds to the mean ± SD of at least three experiments in duplicate.