Reduction of CD16 expression and viral replication in CD14+ monocytes during <i>in vitro</i> minocycline treatment.

<p>CD14+ monocytes were infected with SIVmac316STOP virus and cultured with M-CSF in the presence or absence of minocycline for 24 and 72 hours. With M-CSF treatment, all monocytes expressed CD14 and CD16 prior to minocycline treatment. By flow cytometry, monocytes were first gated based on size (FSC) and granularity (SSC). From this gate HLA-DR+ CD14+ monocytes were selected (<b>A</b>) and CD16 expression on these cells between treatment groups was compared. Histograms represent the median fluorescence intensity (MFI) of CD16 from one representative experiment out of three (<b>B</b>). Averages of MFI ± standard error of the mean in a given treatment group are indicated in the upper left hand corner of the graphs. CD16 expression was significantly higher on untreated than 20 µM minocycline treated monocytes at 48 hours pi (<i>p</i> = 0.021). Untreated monocytes had significantly higher CD16 expression than both 10 µM and 20 µM treated cells at 96 hours pi (<i>p</i> = 0.001). After 96 hours of infection, SIV-p27 was reduced with minocycline treatment (<b>C</b>), with significant differences between control and 20 µM minocycline (<i>p</i> = 0.039). Studies presented here are the results of n = 3 three independent experiments with n = 3 animals per experiment performed in triplicate wells. P values were determined using a Mann-Whitney U test.