Increased levels of aPKC cause morphogenesis defects.

<p>(A) <i>arm^F1a</i>, <i>zw3</i> maternal only mutants (M) display mild patterning defects, but denticles are still present in proper rows. (B) <i>arm^F1a</i>, <i>zw3</i> maternal mutants that express the daGAL4>UAS-aPKC^ΔN transgene display severe defects, as denticle organization is completely lost. (C) Schematic of <i>arm^F1a</i>, <i>zw3</i> maternal mutants expressing the daGAL4>UAS-aPKC^ΔN transgene showing the severe cell shape and alignment defects and random denticle placement (red dots) seen in these embryos. (D–F) Staining of the <i>arm^F1a</i>, <i>zw3</i>maternal mutants expressing aPKC^ΔN with pTyr in red and Arm in green. (D) pTyr shows the random placement of denticle precursors, and the denticle swirls rather than proper rows. (E) Arm localization shows the severe cell shape and alignment defects. (F) Merged view of pTyr and Arm localization shows that denticle placement is random within cells and the dorsal/ventral margins of denticle-producing cells do not display the proper enrichment of Arm protein. Scale bar = 20 µm.