Dendrogram and Principal Component Analysis (PCA) based on Denaturing Gradient Gel Electrophoresis (DGGE) profiles from caecum samples.

<p>A) Dendrogram based on DGGE profiles of PCR amplified 16S rRNA V3 gene region from caecum samples collected from the NOD/Bom mice included in the incidence study. The dendrogram was made using Dice's similarity coefficient and Unweighted Pair Group Method with Arithmetic mean (UPGMA). The scale bar represents the similarity index in percent (100% indicate complete similarity 0% indicate complete dissimilarity). Similarities beyond 92% are defined as being incidental illustrated with a cut of line at 92%. Caecum sample analysis from each individual mouse is marked with either a dot or a star. Stars represent mice from the group of animals treated with 5% ethanol in the drinking water and dots represent mice from the control group receiving pure drinking water. B) Three-dimensional PCA based on DGGE profiles of PCR amplified 16S rRNA V3 gene region from caecum samples collected from the NOD/Bom mice included in the incidence study. Caecum samples representing the individual mice are marked with either a dot or a square. Squares represent mice treated with 5% ethanol in the drinking water and dots represent mice receiving pure drinking water. No grouping according to treatment was observed.